Insemination /Sperm-injection

The eggs retrieved from the follicular fluid is kept in a CO2 incubator for 4-10 hrs for maturation and then 50-100 thousand processed sperm are placed around each egg . In some cases where sperm count are very low, the process of fertilization must be assisted via the injection of individual sperm into each egg, process called ICSI. There are few techniques to process semen and to take highly motile sperm for fertilization. Techniques like “swim-up” technique density gradient and Ficoll entrapment are used to prepare sperm for insemination.

ICSI [Intra Cytoplasmic Sperm Injection]:

Intra Cytoplasmic Sperm Injection (ICSI)

Either Male or female factor where the IVF is not successful, with the help of a special instrument called a micro manipulator, an ICSI is done

Indication for ICSI

  1. Low sperm count
  2. Unexplained failure or poor fertilization in previous IVF cycles
  3. Severe structural abnormalities of sperm
  4. Immotile sperm. Eg. Inherited disorders of the sperm tail for which no other IVF technique is effective (ex: Kartagener’s Syndrome)
  5. Epididymal or testicular sperm obtained from Azoo spermic patients (No sperm count in semen, but normal or low sperm production in the testis)
  6. Sperm obtained by electro ejaculation or from urine incases of retrograde ejaculation
  7. HIV / AIDS, Hepatitis A,B,C affected male partner

Steps of ICSI :

Eggs are first retrieved and placed in a tiny drop in a special dish. While viewing the egg and sperm through a microscope, a single sperm is captured in a fine glass needle and injected through the egg shell and into the center of the egg. Successful fertilization is evaluated the following day. Three to five days after ICSI embryos are transferred into woman’s uterus.

Procedure at Glance:

Alignment of Injecting & holding Pipette

Sperm catching

Micromanipulation Technique

Oocyte holding
Polar body (12 / 6 O’ Clock)

Sperm Injection

Sperm Release