Assisted embryo hatching

 

Assisted Hatching

Definition :

Assisted hatching is an IVF technique in which the zona is treated prior to embryo transfer in order to weaken the wall of the embryo and thus improve the likelihood of successful hatching and embryo implantation.

Indications for Assisted Hatching :

  • Elevated maternal age
  • Elevated follicle stimulating hormone [FSH] level
  • Evidence of a thickened zone pellucida of the embryos in IVF
  • Unexplained implantation failure after two or more embryo transfers.

Why Assisted Hatching :

  • Advanced maternal age
  • Repeated IVF failures
  • Elevated basal FSH
  • Thick Zona pellucida
  • Frozen thowed embryo
  • Excessive fragmentation >20%
Advantages of hatching process :
  • Assisted hatching has demonstrated the potential for improving embryo implantation rates and clinical pregnancy rates in selected patient groups undergoing IVF.

Mechanical Hatching :

Definition :

In this the embryo cover is slit open mechanically with the help of a thin long drawn out glass needle. This is done with the help of a machine called micromanipulator.

Procedure :

Mechanical technique: partial zona dissection :

The zona pellucida is pierced with a very thin glass micro needle through both sides, the needle tip being controlled in the perivitelline space by eye. Then the suction of the holding pipette is stopped and the holding pipette is rubbed against the trapped area of the zona until this area has been completely abraded.